Urinary 5-hydroxyindoleacetic acid by HPLC with electrochemical detection.
نویسندگان
چکیده
منابع مشابه
Direct measurement of homovanillic, vanillylmandelic and 5-hydroxyindoleacetic acids in urine by capillary electrophoresis.
Separation conditions in CE, with a neutral coated capillary and reversed polarity, have been optimised to make direct measurement of vanillylmandelic acid, homovanillic acid and 5-hydroxyindoleacetic acid possible in urine samples without pre-treatment. The method developed has been validated, presenting adequate parameters for linearity, accuracy and precision. Detection limits range from 0.0...
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The analysis of biogenic amines (BA) and pterins in cerebrospinal fluid (CSF) is essential for the early diagnosis of neurotransmission defects in the paediatric age. Our aim was to standardize previously reported HPLC procedures for the analysis of BA and pterins in CSF and to establish reference values for a paediatric population. Samples from 127 subjects (age range 11 days to 16 years; aver...
متن کاملUniform chromatographic conditions for quantifying urinary catecholamines, metanephrines, vanillylmandelic acid, 5-hydroxyindoleacetic acid, by liquid chromatography, with electrochemical detection.
Uniform liquid-chromatographic conditions were developed such that we could quantify norepinephrine, epinephrine, normetanephrine, metanephrine, vanillylmandelic acid, and 5-hydroxyindoleacetic acid in urine by using a single mobile phase of monochloroacetic acid and citric acid, 0.1 mol/L each. All compounds were separated on a C18 column and detected electrochemically at a potential of +0.800...
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7. Knight JA, Haymond RE. Improved colorimetry of urinary 3methoxy-4-hydroxyphenylaceticacid (homovanillic acid). Cliii Chem 1977;23:2007-10. 8. Ruthven CRJ, Sandler M. An improved method for the estimation of homovanullicacid in human urine. Clin Chiin Acts 1966;14:511-8. 9. Udenfriend S, Titus E, Weisabach H. The identification of 5hydroxy-indoleacetic acid in normal urine and a method for it...
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ورودعنوان ژورنال:
- Clinical chemistry
دوره 35 3 شماره
صفحات -
تاریخ انتشار 1988